N0577-15A
NEDD4 (E3 Ubiquitin-protein Ligase NEDD4, Cell Proliferation-inducing Gene 53 Protein, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 4, NEDD-4, KIAA0093, NEDD4-1, PIG53)
Size
£819.00
- SKU:
- N0577-15A
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- E. coli-derived, recombinant protein corresponding to Asn630-Gly768 from human NEDD4.
- Format:
- Supplied as a lyophilized powder from a PBS, 5% trehalose. Reonstitute with 500ul sterile PBS.
- Extra Details:
- NEDD4 (Neural precursor cell-expressed developmentally down-regulated gene 4-1; also RPF1) is a 115-120kD member of the HECT E3 ubiquitin ligase family of proteins. The NEDD4 gene is widely expressed, and serves as a complement to a related gene product termed NEDD4-2/NEDD4L. Both molecules ubiquitinate multiple proteins. NEDD4-1 acts preferentially on PTEN, tumor suppressors and endocytic proteins, while NEDD4-2 acts preferentially on membrane transporters. Human NEDD4-1 is 900aa in length and contains one PKC region (aa20-124), four WW domains (aa196-505) and one E3 HECT domain (aa543-897). Caspase cleavage after Asp197 generates a 95kD C-terminal, and smaller but variable sized N-terminal fragment. There are multiple splice forms. Two show a 516aa substitution for aa1-169 and 1-97, respectively, while a third shows the latter substitution coupled to a deletion of aa170-185. Over aa211-349, human NEDD4-1 shares 83% and 72aa identity to mouse and rat NEDD4-1 and less than 25aa identity with human NEDD4-2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.25ug/ml with jurkat human acute T cell leukemia cell line, HT‑2 mouse T cell line, and NRK rat normal kidney cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice