N0571-55D
Nectin 3 (Nectin-3, CD113, Poliovirus Receptor-related Protein 3, PRR3, Pvrl3)
Size
£1,088.00
- SKU:
- N0571-55D
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant corresponding to aa50-400 from the extracellular domain of mouse Nectin 3, fused to IgG Fc.
- Clone:
- 103-A1
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
- Extra Details:
- Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin/PRR-family, also described as the alpha herpes virus receptor family consists of four members, nectin 1, 2, 3 and 4. All the members of the nectin family have two or three slice variants. For nectin 3; nectin 3alpha, 3beta and 3gamma isoforms exist. Both nectin 2 and nectin 3 are ubiquitously expressed whereas nectin 1 is abundantly expressed in brain. Nectin 2 or 3 is expressed in cells where cadherin are not expressed, such as blood cells and spermatids. Nectin 3 is a transmembrane protein that is mainly expressed in testis and placental tissues. Nectin 3/PRR3 interacts in vivo with both long and short isoforms of afadin. Applications: Suitable for use in ELISA, Flow Cytometry, Immunoprecipitation, Functional Assays and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1ug/1x10e6 cells Immunohistochemistry (Frozen): 1:10 Fixation with PFA or acetone/methanol is recommended. Immunoprecipitation: 1-2ug/100-500ug of total protein (1ml of cell lysate) Indirect ELISA: 0.5ug/ml mouse nectin-3 coated per well. Positive signal with 2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: L-cells (A cultured line of C3H mouse fibroblasts), Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice