N0547-09

NCBP2, Recombinant, Human, aa1-156, His-Tag (Nuclear Cap-binding Protein Subunit 2, 20kD Nuclear Cap-binding Protein, Cell Proliferation-inducing Gene 55 Protein, NCBP 20kD Subunit, CBP20, NCBP-interacting Protein 1, NIP1, CBP20, PIG55)

Size

£512.00

SKU:: N0547-09
Molecular Weight:: 20.1
Purity:: ≥95% (SDS-PAGE).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20% glycerol, 0.1M sodium chloride, 1mM DTT.
Extra Details:: NCBP2, also known as CBC2, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. This protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Source: Recombinant corresponding to aa1-156 of human NCBP2 protein, fused to His-tag at N-terminus, expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNQ Molecular Weight: 20.1kD (176aa) confirmed by MALDI-TOF Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.