N0023-01

NAP2 (Neutrophil Activating Protein 2, CXCL7, PBP (parent molecule), CTAP-III (precursor))

Size

£466.00

SKU:: N0023-01
Purity:: Purified by Protein G affinity chromatography from cell culture.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human NAP2 expressed in E. coli.
Clone:: 3H428
Format:: Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 500ul sterile ddH2O.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (Sandwich): 2-4ug/ml using 100ul/well antibody solution) In conjunction with N0023-03 as a detection antibody, allows the detection of 0.5-1ug/ml of recombinant human NAP-2. Western Blot: 0.5-1ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human NAP-2 is 0.5-1ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.