N0021-05A
NKD2 (Protein Naked Cuticle Homolog 2, Naked-2, hNkd2)
Size
£448.00
- SKU:
- N0021-05A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to C-HKRYRQKGREGHS, from NKD2, at the internal region of the protein (NP_149111.1).
- Format:
- Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
- Extra Details:
- Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism. Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif; however, Drosophila Naked binds to zinc instead of calcium. Naked inhibits the canonical Wnt/B-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway. Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/B-catenin pathway. Naked2 is myristoylated and is required for sorting of TGF-alpha to the basolateral plasma membrane of polarized epithelial cells. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice