Natural Resistance Associated Macrophage Protein (Nramp1)

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£754.00

SKU:: N0020-01K
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated synthetic peptide mapping to a fragment of residues within 21 amino acids in the C-terminal, cytoplasmic domain of mouse Nramp1. Species Sequence Homology: bison, bovine, sheep, red deer, 59%; human, 57%.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Extra Details:: Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Naturally occurring and experimentally induced mutations at Nramp1 abrogate natural resistance to infection. Polymorphic variants at the human Nramp1 locus are associated with differential susceptibility of humans to tuberculosis and leprosy. Nramp1 is expressed in the lysosomal compartment of monocytes and macrophages, lungs, and spleen. It is induced in response to lymphokines and bacterial products. After phagocytosis, Nramp1 is targeted to the membrane of the microbe-containing phagosome, where it may modify the intraphagosomal milieu to affect microbial infection. Although the physiological role of Nramp1 is unclear, structural predictions suggest that Nramp1 encodes the prototypic member of a transporter family. Nramp1 (human 550aa, chromosome 2q35; mouse 548aa) protein is highly hydrophobic integral membrane glycoproteins composed of 12-transmembrane (TM) domains that possess several structural characteristics of ion channels and transporters. Both N- and C-termini are predicted to be cytoplasmic. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. See N0020-01M. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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