Natural Resistance Associated Macrophage Protein (Nramp2)

Size

£754.00

SKU:: N0020-01A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 19 aa synthetic peptide sequence located at the predicted cytoplasmic loop #4 of rat NRAMP2 was synthesized, coupled to KLH. Species sequence homology: 100% mouse, 94% human, 73% zebra fish NRAMP2.
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL technique ELISA: 0.5-1ug/ml Use 1ug/ml control peptide/well Immunohistochemistry: 5-20ug/ml. Use paraformaldehyde fixed sections. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: N0020-01: Natural Resistance Associated Macrophage Protein, Rat (Nramp2) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice