N0019-60B
Natriuretic Peptide Receptor A (NPR-A)
Size
£825.00
- SKU:
- N0019-60B
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- NS0-derived recombinant mouse Natriuretic Peptide Receptor A extracellular domain (aa29-469) (UniProt # P18293). Species Sequence Homology: rat (97%), human (86%).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Mouse Natriuretic Peptide Receptor-A (NPR-A), also known as NPR-1, ANP-R1 or guanlyl cyclase-A, is a 119 kD member of the guanylyl cyclase family of proteins. NPR-1 is a 1029 amino acid type I transmembrane glycoprotein that contains a 441 amino acid extracellular domain and a 567 amino acid cytoplasmic region. The cytoplasmic region shows a tyrosine kinase and guanylyl cyclase catalytic domain. NPR-1 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B-type n.p.) ANP binding induces bronchodilation and a reduction in blood pressure. Over the extracellular domain, mouse NPR-1 is 97% and 86% identical to rat and human NPR-1, respectively. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml. Detection limit is ~10ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1ug/ml. Detection limit is ~0.5ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice