N0019-40

Nanos2 (Nanos homolog 2)

Size

£787.00

SKU:: N0019-40
Application:: WB
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Met1-Arg138 from human Nanos2, expressed in E. coli.
Clone:: 8H151
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Nanos2 is a cytoplasmic RNA-binding protein that belongs to the Nanos family of translational repressors. The 138 amino acid (aa) protein contains a 55 aa nanos-type zinc finger domain with two C2HC motifs. Human and mouse Nanos2 share identity 75% aa sequence identity overall but 95% within the zinc finger RNA binding domain. In the mouse, Nanos2 is located in male germ cells and is essential for spermatogenesis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml with recombinant human Nanos2 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.