Nak1 (Nur77, NR4A1)

Size

£594.00

SKU:: N0018
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Partial synthetic peptide corresponding to an internal portion of the human NGFI-B alpha protein (between amino acids 200-300)
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis. Nak1 is a phosphoprotein and its size ranges from 67kD to 88kD, depending on post-translational modifications. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000; 84kD band observed Optimal dilutions to be determined by the researcher. Recommended Positive Control: Molt-4 or NIH3T3 whole cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.