N0018-70B

NLRP10 (NACHT, LRR and PYD Domains-containing Protein 10, Nucleotide-binding Oligomerization Domain Protein 8, NALP10, NOD8, PYNOD)

Size

£825.00

SKU:: N0018-70B
Application:: WB
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa386-655 from human Pynod expressed in E. coli (Q86W26) Species sequence homology: mouse and rat (50%).
Clone:: 11n133
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Pynod, is a 75kD cytoplasmic protein that associates with the ASC component of inflammasomes. Pynod inhibits ASC-dependent apoptosis, NFkB activation, and Caspase-1-dependent processing of IL-1b. Human Pynod contains one Pyrin domain aa1-96 and one NACHT domain aa167-484 but no leucine-rich repeats. Within aa386-655, human Pynod shares ~50% aa sequence identity with mouse and rat Pynod. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.