N0018-64P

NALP3 (NACHT, NLRP3, C1orf7, CIAS1, LRR and PYD Domains-containing Protein 3, Angiotensin/Vasopressin Receptor AII/AVP-like, Caterpiller Protein 1.1, PYPAF1, CLR1.1, Cold Autoinflammatory Syndrome 1 Protein, Cryopyrin, PYRIN-containing APAF1-like Protein 1)

Size

£825.00

SKU:: N0018-64P
Application:: ICC
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Ala4-Val223 from human NALP3, expressed in E. coli.
Clone:: 12K139
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: NALP3 also known as CIAS1, PYPAF or Cryopyrin is a cytosolic ~120kD member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NALP3 activates caspases 1 and 5. Defects in NALP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Human NALP3 contains an N-terminal Pyrin domain aa1-93 followed by a Nacht region aa220-536, and seven LRRs aa740-991. Within the sequence used as an immunogen, human NALP3 shares 76% and 75% aa identity with mouse and rat NALP3, respectively. Isoforms of 979, 979, 922 and 719aa lack aa720–776, aa836-892, both regions, or terminate at aa719. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml using immersion fixed Jurkat human acute T cell leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice