N0018-60H
NALP1 (NACHT-, LRR- and PYD-Containing Protein 1, DEFCAP, NAC, CARD7, Caspase Recruitment Domain 7)
Size
£655.00
- SKU:
- N0018-60H
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 13aa near the C- terminus of human NALP1.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
- Extra Details:
- NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: U937 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice