N0002-45
NAALADase-Like 2 (N-acetylated A-linked Acidic Dipeptidase Like 2, NAALADL2)
Size
£863.00
- SKU:
- N0002-45
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- NS0-derived, recombinant human NAALADL2. (aa 152 - 795)
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- NAALADL2 (N-acetylated a-linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444-596) and a TfR-like dimerization region (aa 688-777). There are multiple splice forms. One shows a 4 aa substitution for aa 292-795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314-795, and a third contains an alternate start site at human NAADADL2 is 87% and 82% aa identical to mouse and canine NAADADL2, respectively. Applications: Suitable for use in Flow Cytometry, Direct ELISA, Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: use 10ul to label 10e6 cells. Direct ELISA: 0.5 -1ug/mL detection limit for rhNAALADL2 ~ 0.3ng/well. Western Blot: 0.1-0.2ug/ml detection limit for rhNAALADL2 ~ 10ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10ug/mL Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice