M9925-01C
MYPT1 (Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, MYPT1, Protein phosphatase 1 regulatory subunit 12A, Protein phosphatase myosin-binding subunit)
Size
£581.00
- SKU:
- M9925-01C
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to N-terminal residues of human MYPT1. Species Sequence Homology: chicken, bovine, canine, porcine and zebra fish
- Format:
- Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
- Extra Details:
- Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist. The myosin phosphatase holoenzyme is composed of three subunits: the PP1 catalytic subunit (PP1c), a targeting/regu- latory subunit (MYPT/myosin-binding subunit of myosin phosphatase) and a 20 kD subunit of unknown function (M20). MYPT binding to PP1c delta alters the conforma- tion of the catalytic cleft and increases enzyme activity and specificity. Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed while MYPT2 expression appears to be exclusive to heart and brain. Related family members also include MBS85, MYPT3 and TIMAP. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice