Myosin (Cardiac) Light Chain (MLC-I, Essential Myosin Light Chain Isoform)

Size

£550.00

SKU:: M9850-02A
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant human myosin (cardiac) light chain fragment.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Reconstitute with 50ul sterile ddH2O.
Extra Details:: Human ventricular myosin light chain (MLC-I, Essential Myosin Light Chain Isoform) is a 21kD cardiac contractile protein, which is one of component of myosin structure. The protein MLC-I is present in the contractile apparatus and not in cytosol. In severe congestive heart failure (CHF), myofibrils may degenerate and subsequently, the myofibrillar components may be released into the circulation. The increased blood levels of MLC-I could indicate morphological changes in CHF, myocardial infarction, structural abnormalities in viable myocytes also observed in non-ischaemic CHF, myocardial necrosis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.