M9848-03J

Myosin Light Chain 2, aa1-166, His-Tag, Recombinant, Human (MYL2, MLC2, CMH10)

Size

£512.00

SKU:: M9848-03J
Molecular Weight:: 20.9
Purity:: ≥95% by SDS PAGE. Purified by using conventional chromatography techniques.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris pH 8.0, 20% glycerol, 5mM CaCl2.
Extra Details:: Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. Recombinant protein corresponding to aa1-166 from human MYL2, fused to His-tag at N-terminus, expressed in E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.