M9790-02X

Myogenin (BSA & Azide Free)

Size

£1,298.00

SKU:: M9790-02X
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein containing rat myogenin aa30-224. MW of Antigen: 34kD Epitope: aa138-158 Cellular Localization: Nuclear
Clone:: 3F282
Format:: Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See M9790-02
Extra Details:: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas. Applications: Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Gel Supershift: 1mg/ml Immunoprecipitation (Native verified) (Use Protein G): 2ug/mg protein lysate Western Blot: 1-2ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin):Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: Rh30 cells, Rhabdomyosarcoma or Wilm’s tumors. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice