M9790-02
Myogenin
Size
£1,106.00
- SKU:
- M9790-02
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein containing rat myogenin aa30-224. MW of Antigen: 34kD Cellular Localization: Nuclear. Epitope: aa138-158.
- Clone:
- 3F282
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M9790-02X
- Extra Details:
- Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Native verified): 2ug/mg protein lysate. Use Protein G. Western Blot: 1-2ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 1:50 for 20 minutes at RT using the LP system, for 30min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Rh30 cells, Rhabdomyosarcoma or Wilm’s tumors. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice