M9760-01E

Myeloperoxidase (MPO, pANCA) (PE)

Size

£730.00

SKU:: M9760-01E
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human myeloperoxiase.
Clone:: 3H2762 (2C7)
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml ddH2O.
Extra Details:: Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry (Neat): Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Detects myeloid cells following membrane permeabilization. Optimal working dilutions to be determined by researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Recommended Negative Controls: I1904-79D IgG1 Murine Negative Control (PE) Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.