M9760-01A-ML650
Myeloperoxidase (MPO, pANCA) (MaxLight 650)
Size
£940.00
- SKU:
- M9760-01A-ML650
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human Myeloperoxidase.
- Clone:
- 3H2762 (2C7)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulfide bond. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, FLISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100. Membrane permeabilization is required. Immunohistochemistry (Frozen): 1:1000-1:5000 Immunohistochemistry (Paraffin): 1:500-1:1000 Western Blot: Non-reducing conditions. Optimal working dilutions to be determined by researcher. Recommended Secondary Reagent (FC): I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Recommended Positive Control Tissue (IHC): Bone marrow Recommended Secondary Antibodies (IHC): I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb x Mo Hybridoma: Spleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice