M9757-50B
Myelin-Associated Glycoprotein, Small/Large (S/L-MAG)
Size
£793.00
- SKU:
- M9757-50B
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Human S/L-MAG fusion protein with a C-terminal region insert.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- The Myelin-Associated Glycoprotein (MAG) is a minor constituent of the myelin sheath in the central nervous system (CNS) and the peripheral nervous system (PNS). MAG is a member of the immunoglobulin superfamily and shares significant homology with the neural cell adhesion molecule (N-CAM). In rat there are two isoforms of MAG that are the result of alternative splicing of the primary MAG. Large-MAG (L-MAG) has been identified as the functionally relevant isoform in the central nervous system, while small-MAG (S-MAG) maintains the integrity of myelinated fibers in the peripheral nervous system. L-MAG has a non-glycosylated mass of 72kD, whereas the glycosylated form has an apparent molecular weight of 100kD Small-MAG (S-MAG) has a non-glycosylated apparent molecular weight of 67kD. MAG is an inhibitor of axon regeneration and has an important role in maintaining a stable interaction between axons and myelin. Early loss of MAG in the development of multiple sclerosis plaques suggests its role in the pathogenesis of this disease. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunoprecipitation: 1-3ug/IP reaction Immunofluorescence: 10-30ug/ul Optimal dilutions to be determined by the researcher. Positive Control: Rat brain homogenates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice