Myelin-Associated Glycoprotein, Small/Large (S/L-MAG) (FITC)

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£1,049.00

SKU:: M9757-50B-FITC
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human S/L-MAG fusion protein with a C-terminal region insert.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: The Myelin-Associated Glycoprotein (MAG) is a minor constituent of the myelin sheath in the central nervous system (CNS) and the peripheral nervous system (PNS). MAG is a member of the immunoglobulin superfamily and shares significant homology with the neural cell adhesion molecule (N-CAM). In rat there are two isoforms of MAG that are the result of alternative splicing of the primary MAG. Large-MAG (L-MAG) has been identified as the functionally relevant isoform in the central nervous system, while small-MAG (S-MAG) maintains the integrity of myelinated fibers in the peripheral nervous system. L-MAG has a non-glycosylated mass of 72kD, whereas the glycosylated form has an apparent molecular weight of 100kD Small-MAG (S-MAG) has a non-glycosylated apparent molecular weight of 67kD. MAG is an inhibitor of axon regeneration and has an important role in maintaining a stable interaction between axons and myelin. Early loss of MAG in the development of multiple sclerosis plaques suggests its role in the pathogenesis of this disease. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunoprecipitation: 1-3ug/IP reaction Immunofluorescence: 10-30ug/ul Optimal dilutions to be determined by the researcher. Positive Control: Rat brain homogenates Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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