Myelin-Associated Glycoprotein, Small/Large (S/L-MAG) (Biotin)

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£1,049.00

SKU:: M9757-50B-BIOTIN
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human S/L-MAG fusion protein with a C-terminal region insert.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Extra Details:: The Myelin-Associated Glycoprotein (MAG) is a minor constituent of the myelin sheath in the central nervous system (CNS) and the peripheral nervous system (PNS). MAG is a member of the immunoglobulin superfamily and shares significant homology with the neural cell adhesion molecule (N-CAM). In rat there are two isoforms of MAG that are the result of alternative splicing of the primary MAG. Large-MAG (L-MAG) has been identified as the functionally relevant isoform in the central nervous system, while small-MAG (S-MAG) maintains the integrity of myelinated fibers in the peripheral nervous system. L-MAG has a non-glycosylated mass of 72kD, whereas the glycosylated form has an apparent molecular weight of 100kD Small-MAG (S-MAG) has a non-glycosylated apparent molecular weight of 67kD. MAG is an inhibitor of axon regeneration and has an important role in maintaining a stable interaction between axons and myelin. Early loss of MAG in the development of multiple sclerosis plaques suggests its role in the pathogenesis of this disease. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunoprecipitation: 1-3ug/IP reaction Immunofluorescence: 10-30ug/ul Optimal dilutions to be determined by the researcher. Positive Control: Rat brain homogenates Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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