MyD88, CT (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene, Myeloid Differentiation Primary Response Protein MyD88)

Size

£655.00

SKU:: M9755
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 17aa near the C-terminal of human MyD88. Epitope: within the last 50aa. Species Sequence Homology: porcine, sheep, bovine and chicken
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members of the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues. Specificity was further verified by MYD88 specific knockout. MyD88 signal was not detected in MyD88 knockout HeLa cells as compared to that in control wild type cells. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml. Immunofluorescence (IC): 10-20ug/ml. Immunohistochemistry (paraffin): 10-20ug/ml. Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer, pH 6.0, is recommended. Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysate from Jurkat cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice