MyD88, IN (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene, Myeloid Differentiation Primary Response Protein MyD88)

Size

£655.00

SKU:: M9755-10
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 16aa near the center of human MyD88. Epitope: aa220-270. Species Sequence Homology: porcine, sheep, bovine and chicken
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members of the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues. Applications: Suitable for use in Immunoprecipitation, Immunofluorescence, Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1-2ug/ml. Immunofluorescence: 10-20ug/ml. Immunohistochemistry (paraffin): 2ug/ml. Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer, pH 6.0, is recommended. Optimal dilutions to be determined by the researcher. Positive Control: A431 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice