M9601-30D
Myc-Tag (c-myc, myc-1 epitope tag)
Size
£863.00
- SKU:
- M9601-30D
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- KLH-conjugated, synthetic peptide corresponding to aa410-420 (MEQKLISEEDL) of human Myc.
- Clone:
- 4A6
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- The oncogene v-myc was first identified as the transforming gene of the avian myelocytomatosis retrovirus MC29 (hence the name myc). Five members of the human myc family of nucleus-associated phosphoproteins (c-, N-, L-, B-, and R-myc) are currently known. The N-myc gene gives rise to at least two nuclear phosphoproteins of 64kD and 67kD with a relatively short half life (30 minutes) in vivo and exhibit DNA binding properties in vitro. Amplification of the N-myc gene has been reported in human neuroblastomas and cell lines. For example, amplification of the N-Myc gene is associated with a poor prognosis in some neuroblastomas. Myc proteins are potent transcriptional activators that regulate gene expression by forming heterodimers with Max and binding specifically to CAC(G/A)TG sequence motifs in target genes. Max proteins can also form homodimers that recognize the same DNA motif as the Myc/Max heterodimer. Max homodimers, however, repress transcription. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice