M9600-04-ML650
Myb-binding protein 1A (MYBBP1A, p160) (MaxLight 650)
Size
£991.00
- SKU:
- M9600-04-ML650
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from the C-terminal region of the human MYBBP1A
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The Myb family of transcription factors, which includes the structurally related A-, B-, and c-Myb genes, regulate differentiation, cellular growth and transactivating gene expression. c-Myb plays an essential role in controlling the proliferation and differentiation of hematopoietic cells, cellular levels of c-Myb decreasing as cells reach terminal differentiation. Myb-binding protein 1A (MYBBP1A) is a novel nuclear protein localized predominantly, but not exclusively, in nucleoli. Although initially isolated as a c-Myb-interacting protein, MYBBP1A is expressed ubiquitously, and associates with a number of different transcription factors. MYBBP1A associates with the aromatic hydrocarbon receptor (AhR), enhancing transactivation and increasing AhR-dependent gene expression. MYBBP1A is a powerful negative regulator of PPAR gamma coactivator 1 alpha (PGC-1 ) by p38 MAPK and also acts as a nucleocytoplasmic shuttling protein that utilizes CRM1-dependent and independent nuclear export pathways. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/mL Optimal dilution to be determined by the researcher. Positive Controls: Human HeLa, TF-1, JEG-3 and HEK293 cell lysates. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice