M9581

Mva1269I (BsmI)

Size

£294.00

SKU:: M9581
Storage Conditions:: -20°C
Supplier:: United States Biological
Extra Details:: 5'-G A A T G C N↓-3' 3'-C T T A C↑G N-5' Concentration: ~10u/ul Source: Micrococcus varians RFL1269 Unit Definition: One unit is defined as the amount of Mva1269I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Incubation Temperature: 37°C Storage Buffer: Supplied as a liquid in 10mM Tris-HCl pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Dilution Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid containing 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-04: Restriction Enzyme Buffer E, 10X: Supplied as a liquid in 10mM Tris-HCl, pH 8.5, 10mM magnesium chloride, 100mM potassium chloride, 0.1mg/ml BSA. Thermal Inactivation: Mva1269I is inactivated by incubation at 65°C for 20min. Enzyme Properties: Methylation Effects: Dam, Dcm, EcoKI: Never overlaps - no effect CpG: May overlap - no effect EcoBI: May overlap - effect not determined Stability during Prolonged Incubation: A minimum of 0.1units of Mva1269I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Digestion of Agarose-embedded DNA: A minimum of 5 units of Mva1269I is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 46 PhiX174: 4 pBR322, pUC57, M13mp18/19: 1 pUC18/19, pTZ19R/U: 0 Thermal Inactivation: Mva1269I is inactivated by incubation at 65°C for 20min. Enzyme Properties: Methylation Effects: Dam, Dcm, EcoKI: Never overlaps - no effect CpG: May overlap - no effect EcoBI: May overlap - effect not determined Stability during Prolonged Incubation: A minimum of 0.1units of Mva1269I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Digestion of Agarose-embedded DNA: A minimum of 5 units of Mva1269I is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 46 PhiX174: 4 pBR322, pUC57, M13mp18/19: 1 pUC18/19, pTZ19R/U: 0

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