M9557-03
mutY
Size
£754.00
- SKU:
- M9557-03
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 19-aa peptide near the C-terminus of human mutY (KLH).
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
- Extra Details:
- Escherichia coli MutY is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. The N-terminal domain of MutY, residues 1-226, has been shown to retain catalytic activity. The post-replicative adenine:8-oxo-7,8- dihydroguanine (GO) mismatch is crucial for G:C to T:A transversion. This mismatch is corrected by Escherichia coli MutY which excises the adenine from A:GO. A candidate gene coding for the human counterpart of MutY has been cloned as hMYH (535 aa) (1). Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications have not been tested. Recommended Dilution: ELISA: 1:10,000-50,000. Using 50-100ng control peptide/well. Western Blot: 1:1000-1:5000. Optimal dilutions to be determined by the researcher. Control Peptide: M9555-01 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice