M9520-23H
Muscarinic Acetylcholine Receptor M3 (CHRM3, HM3)
Size
£825.00
- SKU:
- M9520-23H
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human CHRM3 expressed in NS0 cells. Species Sequence Homology: mouse and rat, 92%
- Clone:
- 11D4
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Muscarinic acetylcholine receptor M3 (CHRM3) is a 65kD 7-transmembrane glycoprotein that is predominantly expressed in the central nervous system, smooth muscle, and heart. CHRM3 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Applications: Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 8-25ug/ml on immersion fixed paraffin-embedded sections of human brain (cortex) tissue. Flow Cytometry: 0.25ug/10e6 using HEK293 human cell line transfected with human CHRM3 and eGFP. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice