M9520-13B-APC

Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007) (APC)

Size

£1,070.00

SKU:: M9520-13B-APC
Purity:: Purified immunoglobulin
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.
Clone:: 2Q147 (M2-2-B3)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Applications: Suitable for use in Immunohistochemistry and Immunocytochemistry. Does not work on paraffin embedded tissues, and Immunoblotting. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (4% paraformaldehyde fixed tissue): 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. IMMUNOHISTOCHEMISTRY PROTOCOL Use on 30 um, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. 1. 3 x 10 minute washes in TBS (with or without 0.25% Triton). 2. Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody). 3. Incubate M9520-13B diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at RT followed by 16 hours at 4°C. 4. 3 x 10 minute washes in TBS. 5. Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody). 6. 3 x 10 minute washes in TBS. 7. ABC Elite (1:200) in TBS. 8. 2 x 10 minute washes in TBS. 9. 1 x 10 minute wash in phosphate buffer (no saline). 10. DAB reaction with 0.06% NiCl added for intensification. 11. 2 x 10 minute washes in PBS. 12. 1 x 10 minute wash in phosphate buffer (no saline). Note: Applications are based on unconjugated antibody.

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