M5004-98
Major Urinary Protein (MUP) (Not for Export EU)
Size
£466.00
- SKU:
- M5004-98
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Purified mouse major urinary proteins isolated from pooled normal urine
- Format:
- Supplied as a lyophilized powder. No preservatives added. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
- Extra Details:
- The MUP is a sex related complex with a pre-albumin mobility in gel electrophoresis. Concentrations in male mice are between 50 to 150mg/ml, in female mice 10 to 20mg/ml. The complex is also detectable in serum, however concentrations are about 200 to 400 times lower. Variations in the composition of the complex are genetically determined. Applications: Suitable for use in Immunoelectrophoresis and single or double radial immunodiffusion (Mancini, Ouchterlony). Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 120ul against 2ul urine, plasma or equivalent Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 2ul serum samples (neat and diluted) in 2mm diameter peripheral wells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice