MUCDHL (Mucin and Cadherin-Like Protein, mu-Protocadherin)

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£863.00

SKU:: M4703-11
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Purified, NS0-derived rmMUCDHL (rmMUCDHL; aa 29 - 479; Accession # NP_082345)
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Mucin and cadherin-like protein (MUCDHL), also known as mu-protocadherin, is a 100kD type I transmembrane protein belonging to the protocadherin family of cell adhesion molecules. It is found apically on the brush border of renal proximal tubule epithelial cells. The extracellular domain of mature mouse MUCDHL contains four cadherin domains plus three repeat regions that constitute a mucin stalk (aa 541-614). At least one splice variant exists in mouse that shows a deletion of aa's 459-620. Over aa's 29-479, mouse MUCDHL shares 63% and 79% aa identity with human and rat MUCDHL, respectively. Applications: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications not tested. Recommended Dilution: Western Blot: Can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 1 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Tested in flow cytometry using primary kidney cells. Dilute this antibody to 25ug/ml and add 10 μL of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: Can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 0.5 ng/well. Optimal dilutions to be determined by the researcher. Reconstitution: Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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