M4701-05N

Mucin 5AC (MUC5AC, Apomucin, Gastric Mucin, Major Airway Glycoprotein, MUC5, Mucin 5 Subtype AC Tracheobronchial, Mucin 5 Subtypes A and C, Mucin 5 Subtypes A and C Tracheobronchial/gastric, Mucin 5AC Oligomeric Mucus/gel Forming, Tracheobronchial Mucin, TBM, Lewis B Blood Group Antigen, LeB)

Size

£544.00

SKU:: M4701-05N
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
Clone:: 9-13M1
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6,7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands. The epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): Strongly positive on ethanol fixed epithelial cells. Immunohistochemistry (paraffin): Very strong positive reaction with ethanol fixed tissues. On formalin postfixed tissues, a protease pretreatment is recommended. Western Blot: Strongly positive without smercaptoethanol pretreatment of mucin solution. Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice