M4700-05C-ML550
Mucin 1 (MUC1 Glycoprotein, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, CD227, EMA, Episialin, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, Mucin-1, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin) (MaxLight 550)
Size
£1,081.00
- SKU:
- M4700-05C-ML550
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Urinary MUC-1 mucin
- Clone:
- 3H2740 (C595 (NCRC48))
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. Mucin 1 is a breast cancer associated mucin encoded by the Muc-1 gene. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, FLISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Paraffin. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Citrate buffer, pH 6.0 is recommended Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher. IHC Positive Control: Breast carcinoma Recommended Negative Control: I1904-86K: IgG3 Negative Control Hybridoma: Myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice