M4700-05C-BIOTIN

Mucin 1 (MUC1 Glycoprotein, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, CD227, EMA, Episialin, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, Mucin-1, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin) (Biotin)

Size

£1,081.00

SKU:: M4700-05C-BIOTIN
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Urinary MUC-1 mucin
Clone:: 3H2740 (C595 (NCRC48))
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. Mucin 1 is a breast cancer associated mucin encoded by the Muc-1 gene. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Paraffin. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Citrate buffer, pH 6.0 is recommended Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher. IHC Positive Control: Breast carcinoma Recommended Negative Control: I1904-86K: IgG3 Negative Control Hybridoma: Myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C. For long-term storage, aliquot and store at 4°C. Aliquots are stable at 4°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice