M4696-10A
mTOR, phosphorylated (Ser2448) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)
Size
£672.00
- SKU:
- M4696-10A
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR. Species sequence homology: mouse and rat.
- Clone:
- 49F9
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
- Extra Details:
- The mammalian target of rapamycin mTOR, also known as FRAP or RAFT(1-3), is a Ser/Thr protein kinase. mTOR acts as a sensor for ATP and amino acids, balancing the availability of nutrients and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal (6), and transmits a positive signal to p70 S6 kinase, and participates in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cellular growth and homeostasis, and its regulation is frequently altered in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Paraffin and Frozen): 1:100. Citrate/TBST Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice