Metal Transporter Protein (MTP1)

Size

£767.00

SKU:: M4695-50B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide consisting of 19aa sequence located within the predicted cytoplasmic C-terminus of mouse MTP1 (KLH). Sequence Homology: Mouse ferroprotein 1/IREG1: 100%; rat ferroprotein 1: 90%; human and chimp ferroprotein 1: 78% and rabbit MTP1/IREG1/ferroprotein 1: 73%.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Extra Details:: Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml. Coat with 1ug/ml Control Peptide. Western Blot: 1-10ug/ml (ECL) Immunofluorescence: 5-10ug/ml using confocal microscopy. Immunohistochemistry: 5-20ug/ml. Use paraformaldehyde fixed tissues. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: M4695-50A: Metal Transporter Protein, Mouse, Control Peptide (MTP1) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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