M4692-45A

MSK1 (Mitogen and Stress activated protein Kinase1)

Size

£889.00

SKU:: M4692-45A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to human MSK1 (aa1-802) from E. coli.
Format:: Supplied as a liquid in PBS, pH 7.2, 5% trehalose.
Extra Details:: Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2/RSKB have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. Detects endogenous human and mouse MSK1 at 95kD. Immunocytochemistry: 2-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.