M4685

MPM2, Ser/Thr, Pro, phosphorylated (Mitotic Protein Monoclonal#2)

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£832.00

SKU:: M4685
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mitotic human HeLa cell cytosolic lysate.
Clone:: 0.T.181
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Extra Details:: Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho aa-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells. This antibody recognizes a variety of proteins that are phosphorylated during mitosis. Applications: Suitable for use in Western Blot, Immunocytochemistry, ELISA, Immunohistochemistry, Immunoprecipitation and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects mitosis-associated proteins in colcemid treated (0.05ug/ml) human HeLa carcinoma cells. Colcemid-treated HeLa cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with M4685. Proteins were visualized using an IgG (HRP) goat anti-mouse antibody and ECL. Immunocytochemistry: 5-15ug/ml shows positive staining of mitotic cells. ELISA: Used in a non-radioactive, dissociation-enhanced time resolved fluoroimmunoassay (DELPHIA). Active enzymes were used to phosphorylate substrate peptides. The phosphorylated peptides were detected using MPM2 in conjunction with a europium (Eu3+) labeled secondary antibody. The phosphorylated peptides detected include: Serine/Threonine Kinase Substrate Peptide, Recombinant, Human (Biotin). Immunoprecipitation: Reported by an independent laboratory. Optimal dilutions to be determined by the researcher. Positive Control: Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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