M4685-94
Mrc2 (Macrophage Mannose Receptor 2, uPARAP, Endo180)
Size
£876.00
- SKU:
- M4685-94
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- NS0-derived rmMrc2 extracellular domain. (aa 31 - 1413)
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Mrc2 (macrophage mannose receptor 2; also uPARAP/Endo180) is a 180kD member of the mannose receptor family. It is found on fibroblasts, macrophages and endothelial cells. Mrc2 binds and internalizes glycoproteins plus collagen types I and IV. Mouse Mrc2 is a type I transmembrane glycoprotein that is 1479 amino acids (aa) in length. It possesses a 1383 aa extracellular domain (ECD) (aa 31-1479) and a short 45 aa cytoplasmic tail. The ECD contains eight C-type lectin domains plus one fibronectin type II domain (aa 181-229) that binds collagen. There are two splice variants, one which shows an eight aa substitution for aa 173-1479, and a second that shows a 72 aa substitution for aa 982-1479. Over aa 31-1413, mouse Mrc2 shares 91% and 97% aa identity with human and rat Mrc2, respectively. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rmMrc2 is ~ 1ng/well. Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice