M4685-80
Mps1, CT
Size
£800.00
- SKU:
- M4685-80
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- His-tagged fusion protein corresponding to aa3-856 of human Mps1, previously known at TTK/PYK.
- Clone:
- 5H9 (4-112-3)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
- Extra Details:
- Mps-1 is a protein kinase thought to play during the spindle checkpoint to regulation of sister chromatids separation when chromsome alingment is complete. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml of this lot detected Mps1 in HeLa total cell extract. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with M4685-80 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system. Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate Mps1 from HeLa total cell extract. Immunocytochemistry: Reported by an independent laboratory to show positive immunostaining for Mps1 in U2OS or HeLa cells permeabilized with 0.5% Triton-X-100 and fixed with methanol or paraformaldehyde. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice