M4520-01

Monocytes, Macrophages

Size

£762.00

SKU:: M4520-01
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat spleen cells
Clone:: 2Q1782 (ED1)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Applications: Suitable for use in Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot and Radioimmunoassay. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry on frozen sections: 1:50-1:300. Immunohistochemistry on Paraffin embedded tissue (Bouin fixation): 1:50-1:300 (requires pretreatment with trypsin or pronase). Flow Cytometry: 1:100-1:200. Use 10ul to label 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.