M4149-20
Membrin (GS27, Golgi SNARE of 27kD)
Size
£371.00
- SKU:
- M4149-20
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to a portion of rat membrin.
- Format:
- Supplied as a liquid in 25mM borate saline, 100mM boric acid, 75mMNaCl, 5mM EDTA (disodium salt), pH 8.4.
- Extra Details:
- Membrin, also known as GS27 (Golgi SNARE of 27 kD), is a 27 kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport (2). Membrin is a member of a protein complex that consists of cis-Golgi vesicle receptor syntaxin 5 and GS28, v-SNARE rbet1 and rsec22, and rsly1. Immunoprecipitation analysis has demonstrated that there are two subcomplexes within this complex: one contains syntaxin 5 and GOS28 and the other contains syntaxin 5, membrin, rsec22 and rbet1 (3,4). These complexes have been postulated to direct the fusion of ER-derived vesicles with Vesicular tubular cluster (VTC), and the fusion of VTCs to form cis-Golgi compartment (3,4). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot (ECL): 2ug/ml Immunohistochemistry: paraffin Optimal dilutions to be determined by researcher. Positive Controls: Rat Brain Tissue Extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice