M4149-10
Membrin (GS27, Golgi SNARE of 27K)
Size
£501.00
- SKU:
- M4149-10
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to rat membrin fused to His6 tag
- Clone:
- 4E157 (4HAD6)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
- Extra Details:
- Membrin, also known as GS27 (Golgi SNARE of 27K), is a 27kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport (1). GS27 is a member of a protein complex that consists of cis-Golgi vesicle receptor syntaxin 5 and GS28, v-SNARE rbet1 and rsec22, and rsly1. Immunoprecipitation analysis has shown that there are two subcomplexes within this complex: one contains syntaxin 5 and GOS28 and the other one contains syntaxin 5, GS27/membrin, rsec22 and rbet1 (2,3). These complexes are postulated to direct the fusion of ER-derived vesicles with Vesicular tubular cluster (VTC), and the fusion of VTCs to form cis-Golgi compartment (2,3). Applications: Suitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot (ECL): 2ug/mL Immunoprecipitation: 2-4ug/mL Immunocytochemistry: 1:500 (4) Optimal dilutions to be determined by the researcher. Positive Control: HeLa Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice