M4127

MLLC (HRX)

Size

£971.00

SKU:: M4127
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: MBP-tagged recombinant protein corresponding to aa3084-3959 a fragment at the C-terminal of human MLLC. Epitope: MLLC C-terminal fragement
Clone:: 4H354 (9-12)
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Extra Details:: Applications: Suitable for use in ChIP, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-1ug/ml detects MLLC in nuclear extract from K562 cells. K562 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with M4127 (1ug/ml). Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.