M4127-01

MLLN (HRX)

Size

£971.00

SKU:: M4127-01
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: MBP fusion protein corresponding to aa161-356 of human mixed lineage leukemia, MLL.
Clone:: 4H355 (N4.4)
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Extra Details:: Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects MLLN in nuclear extract from K562 cells. K562 nuclear extract was resolved by electrophoresis, transferred to PVDF and probed with M4127-01 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibody: I1904-77B: IgG1 (HRP) Pab GtxMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.