M4015-10
Mitotic Cells
Size
£524.00
- SKU:
- M4015-10
- Purity:
- Purified from culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Total cell lysate of the human bladder carcinoma cell line T24.
- Clone:
- 4A63 (8B3G)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
- Extra Details:
- The life cycle of a eukaryotic cell consists of various phases, two of which can easily be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent’ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S and from G2 to M-phase. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100 Immunocytochemistry: 1:50-1:100 using ABC detection reagent. Optimum dilutions to be determined by researcher. Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice