M3985-06-ML650
Mitochondria (MaxLight 650)
Size
£1,062.00
- SKU:
- M3985-06-ML650
- Purity:
- Purified by ammonium sulfate precipitation from hybridoma supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Surface of intact human mitochondria
- Clone:
- 2Q2230 (113-1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. Applications: Suitable for use in Indirect Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Can also be used for isolation of intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads. Other applications have not been tested. Recommended Dilution: Indirect Immunofluorescence: 1:10-1:50, 30-100ul per slide well Immunohistochemistry (paraffin-embedded tissues): 1:80 Using heart ventricle epithelium pretreated with EDTA, pH 8.0. Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells. Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended. Immunohistochemistry: 1:100. Works best with organic fixatives (acetone, methanol, etc.). 2% PFA for 10-15 minutes. can be used but stronger use of formalin or 4% PFA limits staining. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice